LOCUS       sequence-98431-e         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Lakshminarasimhan M, Maldonado MT, Zhou W, Fink AL, Wilson MA
  TITLE     Structural impact of three Parkinsonism-associated missense 
            mutations on human DJ-1.
  JOURNAL   Biochemistry. 2008 Feb 5;47(5):1381-92. doi: 10.1021/bi701189c. Epub
            2008 Jan 9.
  PUBMED    18181649
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi701189c";
            journalName: "Biochemistry"; date: "2008-02-5- 5"; volume: "47"; 
            issue: "5"; pages: "1381-92"
COMMENT     Sequence Label: M26L DJ-1 pET15b
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat ataccatggg cagcagccat caccatcatc
       61 atcacagcag cggccaggtg ccgcgcggca gccatatggc ttccaaaaga gctctggtca
      121 tcctggctaa aggagcagag gaaatggaga cggtcatccc tgtagatgtc ttgaggcgag
      181 ctgggattaa ggtcaccgtt gcaggcctgg ctggaaaaga cccagtacag tgtagccgtg
      241 atgtggtcat ttgtcctgat gccagccttg aagatgcaaa aaaagaggga ccttatgatg
      301 tggtggttct accaggaggt aatctgggcg cacagaattt atctgagtct gctgctgtga
      361 aggagatact gaaggagcag gaaaaccgga agggcctgat agccgccatc tgtgcaggtc
      421 ctactgctct gttggctcat gaaataggtt ttggaagtaa agttacaaca caccctcttg
      481 ctaaagacaa aatgatgaat ggaggtcatt acacctactc tgagaatcgt gtggaaaaag
      541 acggcctgat tcttacaagc cgggggcctg ggaccagctt cgagtttgcg cttgcaattg
      601 ttgaa
//
