LOCUS       sequence-98456-e         547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression of Park7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Milkovic NM, Catazaro J, Lin J, Halouska S, Kizziah JL, Basiaga S, 
            Cerny RL, Powers R, Wilson MA
  TITLE     Transient sampling of aggregation-prone conformations causes 
            pathogenic instability of a parkinsonian mutant of DJ-1 at 
            physiological temperature.
  JOURNAL   Protein Sci. 2015 Oct;24(10):1671-85. doi: 10.1002/pro.2762. Epub 
            2015 Aug 17.
  PUBMED    26234586
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2762"; 
            journalName: "Protein Sci"; date: "2015-10"; volume: "24"; issue: 
            "10"; pages: "1671-85"
COMMENT     Sequence Label: DJ-1 TEV site pET15b
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..83
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgtttacttt aagacggaga tataccatgg gcagcagcca tcatcatcaa cgtcacagca
       61 gcggcctggt gccgcgcggc agccatatgg aaaacctgta ttttcagtct gcttccaaaa
      121 gagctctggt catcctggct aaaggagcag aggaaatgga gacggtcatc cctgtagatg
      181 tcatgaggcg agctgggatt aaggtcaccg ttgcaggcct ggctggaaaa gacccagtac
      241 agtgtagccg tgatgtggtc atttgtcctg atgccagcct tgaagatgca aaaaaagagg
      301 gaccttatga tgtggtggtt ctaccaggag gtaatctggg cgcacagaat ttatctgagt
      361 ctgctgctgt gaaggagata ctgaaggagc aggaaaaccg gaagggcctg atagccgcca
      421 tctgtgcagg tcctactgct ctgttggctc atgaaatagg ttttggaagt aaagttacaa
      481 cacaccctct tgctaagaga caaaatgatg aatggaggtc attacaccta ctctgagaat
      541 cgtgtgg
//
