LOCUS       sequence-98490-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal 
            tag). Confers resistance to G418..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B,
            Frit P, Calsou P
  TITLE     DNA damage triggers SAF-A and RNA biogenesis factors exclusion from 
            chromatin coupled to R-loops removal.
  JOURNAL   Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 
            10.1093/nar/gku601. Epub 2014 Jul 16.
  PUBMED    25030905
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku601"; journalName: "Nucleic Acids Res"; date: 
            "2014-10-1- 1"; volume: "42"; issue: "14"; pages: "9047-62"
COMMENT     Sequence Label: pEGFP-N1-FUS/TLS-FLAGC
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    45..101
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             491..502
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
       61 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc cgccgccacc atggcctcaa
      121 acgattatac ccaacaagca acccaaagct atggggccta ccccacccag cccgggcagg
      181 gctattccca gcagagcagt cagccctacg gacagcagag ttacagtggt tatagccagt
      241 ccacggacac ttcaggctat ggccagagca gctattcttc ttatggccag agccagaaca
      301 caggctatgg aactcagtca actccccagg gatatggctc gactggcggc tatggcagta
      361 gccagagctc ccaatcgtct tacgggcagc agtcctccta tcctggctat ggccagcagc
      421 cagctcccag cagcacctcg ggaagttacg gtagcagttc tcagagcagc agctatgggc
      481 agccccagag tgggagctac agccagcagc ctagctatgg tggacagcag caaagctatg
      541 gacagcagca aagctataat ccccctcagg gctatggaca gcagaaccag tacaacagca
      601 gcagtggtgg tggaggtgga ggtggaggtg gaggtaacta tggccaagat caatcctcca
      661 tgagtagtgg tggtggcagt ggtggcggtt atggcaatca agaccagagt ggtggaggtg
      721 gcagcggtgg ctatggacag caggaccgtg gaggccgcgg caggggtg
//
