LOCUS       sequence-98602-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) and yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB787
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(64..82)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaagacgtca aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc
       61 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
      121 tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt
      181 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
      241 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
      301 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg
      361 cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
      421 aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt
      481 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
      541 gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct
      601 actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat
      661 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
      721 ccattccatc acgg
//
