LOCUS       sequence-98608-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      286..295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             337..372
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
       61 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      121 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      181 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtccggactc
      241 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcggccac catggctact
      301 caagctgacc tgatggagtt ggacatggcc atggagccgg acagaaaagc tgctgtcagc
      361 cactggcagc agcagtctta cttggattct ggaatccatt ctggtgccac caccacagct
      421 ccttccctga gtggcaaggg caaccctgag gaagaagatg ttgacacctc ccaagtcctt
      481 tatgaatggg agcaaggctt ttcccagtcc ttcacgcaag agcaagtagc tgatattgac
      541 gggcagtatg caatgactag ggctcagagg gtccgagctg ccatgttccc tgagacgcta
      601 gatgagggca tgcagatccc atccacgcag tttgacgctg ctcatcccac taatgtccag
      661 cgcttggctg aaccatcaca gatgttgaaa catgcagttg tcaatttgat taactatcag
      721 gatgacgcgg aacttgccac acgtgcaatt cctgagctga caaaactgct aaacgatgag
      781 gaccaggtgg tagttaata
//
