LOCUS       sequence-98656-e         317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR
            in a Cre dependent manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-FLIM-AKART391A
FEATURES             Location/Qualifiers
     source          1..317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..103
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    174..207
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      228..237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(279..300)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caaattttgt aatccagagg tttgattatc gataagcttt gatatcgaat tcgaacctct
       61 tcgagggaca taacttcgta taatgtatgc tatacgaagt tatgggtcga tggtgagatc
      121 tggactagag ggtcgatggt gatgcttgga tcaaaatagg aagaatccca atgataactt
      181 cgtataaagt atcctatacg aagttatact agaattcggt acccgccgcc accatggtga
      241 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      301 taaacggcca caagttc
//
