LOCUS       sequence-98676-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Zhang Z, Xu J, Koppetsch BS, Wang J, Tipping C, Ma S, Weng Z, 
            Theurkauf WE, Zamore PD
  TITLE     Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 
            ligase and Tudor domains.
  JOURNAL   Mol Cell. 2011 Nov 18;44(4):572-84. doi: 
            10.1016/j.molcel.2011.10.011.
  PUBMED    22099305
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.10"; journalName: "Mol Cell"; date: 
            "2011-11-18- 18"; volume: "44"; issue: "4"; pages: "572-84"
COMMENT     Sequence Label: pUASp-Flag-Myc-Qin-attB
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      296..305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             305..370
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      386..395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             395..424
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             434..463
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             473..502
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             512..541
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             551..580
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             614..643
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    656..680
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattcagtgc acgtttgctt gttgagagga aaggttgtgt gcggacgaat ttttttttga
       61 aaaccggtga tagagcctga accagaaaag ataaaagaag gctataccag ctgggagtac
      121 acaaacagag taagtttgaa tagtaaaaaa aatcatttat gtaaacaata acgtgactgt
      181 gcgttaggtc ctgttcattg atcgaggcct gtctagagaa gcttgttcga atctcgagtg
      241 cgcgcttccg gaggtataca cctaggcggt accactgcag tgaattcgga gctccgccac
      301 catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
      361 cgatgacaag caccggttga gctccgccac catggagcaa aagctcattt ctgaagagga
      421 cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa
      481 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt
      541 gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg
      601 cgacctcacc atggagcaaa agctcatttc tgaagaggac ttgaatcacc ggtatacaag
      661 tttgtacaaa aaagcaggct tcatgattgc cgacgacagt ggaataaagc cctttgatcc
      721 caagtttgtg gcgtccaatc tgcagaatct tctgcgctgc ccaaaatgtt caaaatgttt
      781 tacttactta tacaatacga actctgaaga agacaatcat cgtccctttc tactggggat
      841 gcggtcatac tctctgcgag agctgcttgt ggaaccg
//
