LOCUS       sequence-98683-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta3 
            tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2920"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "335-44"
COMMENT     Sequence Label: pRS424-TUB2 - human TUBB3 Cterminal tail delta K
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..268)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     234..252
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     270..286
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     271..287
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             685..693
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gggcgaattg ggtaccggcc gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc
       61 aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt
      121 tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
      181 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa
      241 tgtaagcgtg acataactaa ttacatgact cgaggtcgac ggtatcgata agcttgatat
      301 cgacgtcttt attttggacc ttaagcttca gattcttctt catcatcttc atacatttca
      361 ccttcttctt cagcagtagc atcttggtat tgttggtatt cgctaaccag atcattcata
      421 ttagattcag cctcagagaa ttccaattcg tccatacctt cactagtata ccagtgcaag
      481 aaagcttttc ttttgaacat agcggaaaat tggtcaccaa ctctcttgaa tagctcttga
      541 atagatgtgg agttagcaat gaaagtagca gccatgtcca aaccttgagg agcgacagaa
      601 cacacagcag tttgcacatt gttggggatc cattccacga aatagtctga gtttttagat
      661 tgcactttat gcatttcatc ttccacctcc ttaacggaaa ctttacctct aaagaaggct
      721 gcaacggtaa ggtatctacc gtttcttgga tcggcagcag ccatcatgtt cttggcatca
      781 aacatttgct gtgttaattc
//
