LOCUS       sequence-98704-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  integrin a5 wild-type headpiece expression (a5 beta-propeller and a5
            thigh domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     integrin a5b1 headpiece
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1/hpa5
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..84
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(112..135)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(160..171)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(235..255)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
       61 ggcaactaga aggcacagtc gaggctgatc agcggtttaa acttaagctt acttttcgaa
      121 ctgggggtgg ctccattgag ctagctcctt ctctagtgct tgtaattccc attccagctg
      181 tgcattttcc ttctcaagtg cctggagctc tttttcgcac tgagcatttt ctccaccttg
      241 gaaataaaga ttctccagtc caccggtcag caagatctga gccttgtcct ctatccggct
      301 cttgctctga taatgtaggg ctggcctgag gccgtggctg tccactgggg cttgggggtc
      361 caaggagaag ttgagagcga tgtgaatcgg cgagagtttg tctcgaaatt ctgactcgtt
      421 cctgaggtag atcttcatct ctctgcaatc ctctcgagcc ccattctgga tgagcagggt
      481 ctgggtcagg gttgcctgcc tggaggccag gaacagtgcc cgccgtaccc ctcccttctg
      541 cttctgccag tccagctgaa gttccactgt gaaaccaatg gagtcagcaa cgtgttttcc
      601 agaagcattg aggcagaagc taaggttgat gcaggccaca gggttcccct ctaagctgca
      661 gctccgctcc tctgggttga acatggcggg gaagatggtg agggaggcac tagcggacac
      721 gatggggcgg cccctgtata ccacagcctt gtccacacca aaggacccca caatcagatc
      781 aggatatcca ttgccatcca ggtctcggcc tcctcgaagg gcagag
//
