LOCUS       sequence-98706-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector with CaMKIIa promoter and upstream CMV promoter 
            expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors 
            changes in membrane voltage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Flytzanis NC, Bedbrook CN, Chiu H, Engqvist MK, Xiao C, Chan KY, 
            Sternberg PW, Arnold FH, Gradinaru V
  TITLE     Archaerhodopsin variants with enhanced voltage-sensitive 
            fluorescence in mammalian and Caenorhabditis elegans neurons.
  JOURNAL   Nat Commun. 2014 Sep 15;5:4894. doi: 10.1038/ncomms5894.
  PUBMED    25222271
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-15"; volume: "5:4894. doi"; pages: " 
            10.1038/ncomms5894"
COMMENT     Sequence Label: pLenti-CaMKIIa-Archer1-EGFP
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      423..432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca cttgtggact
       61 aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg gaacagggtc
      121 cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca gggcaaagag
      181 gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg gggcaacggg
      241 aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag gctcgcgagg
      301 ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa gccggttctc
      361 cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc agcgggggat
      421 ccgccaccat ggaccccatc gctctgcagg ctggttacga cctgctgggt gacggcagac
      481 ctgaaactct gtggctgggc atcggcactc tgctgatgct gattggaacc ttctactttc
      541 tggtccgcgg atggggagtc accgataagg atgcccggga atattacgct gtgactatcc
      601 tggtgcccgg aatcgcatcc gcc
//
