LOCUS       sequence-98720-e         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Sanchez-Rivera FJ, Papagiannakopoulos T, Romero R, Tammela T, Bauer 
            MR, Bhutkar A, Joshi NS, Subbaraj L, Bronson RT, Xue W, Jacks T
  TITLE     Rapid modelling of cooperating genetic events in cancer through 
            somatic genome editing.
  JOURNAL   Nature. 2014 Oct 22. doi: 10.1038/nature13906.
  PUBMED    25337879
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Oct 22. doi: 10.1038/nature13906."
COMMENT     Sequence Label: pSECC
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..137
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
       61 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      121 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      181 aaaggacgaa acaccggaga cggttgtaaa tgagcacaca aaatacacat gctaaaatat
      241 tatattctat gacctttata aaatcaacca aaatcttctt tttaataact ttagtatcaa
      301 taattagaat ttttatgttc ctttttgcaa acttttaata aaaatgagca aaataaaaaa
      361 acgctagttt tagtaactcg cgttgttttc ttcaccttta ataatagcta ctccaccact
      421 tgttcctaag cggtcagctc ctgcttcaat cattttttga gcatcttcaa atgttctaac
      481 tccaccagct gctttaacta aagcattgtc tttaacaact gacttcatta gtttaacatc
      541 ttcaaatgtt gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc
      601 ttcaacagct atttcacaag ctttcatgat ttc
//
