LOCUS       sequence-98727-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Sanchez-Rivera FJ, Papagiannakopoulos T, Romero R, Tammela T, Bauer 
            MR, Bhutkar A, Joshi NS, Subbaraj L, Bronson RT, Xue W, Jacks T
  TITLE     Rapid modelling of cooperating genetic events in cancer through 
            somatic genome editing.
  JOURNAL   Nature. 2014 Oct 22. doi: 10.1038/nature13906.
  PUBMED    25337879
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Oct 22. doi: 10.1038/nature13906."
COMMENT     Sequence Label: pSECC
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctctaaaac agagacgtac aaaaaagagc aagaagctaa aaaagattta aaaattattt
       61 ttagcgcagt taatggaaca ggaactaaat ttaccccaaa aatattacgt gaatcaggat
      121 ataacgttat tgaggttgaa gagcatgcat ttgaagatga aacatttaaa aatgttgtaa
      181 atccaaatcc agaatttgat cctgcatgaa aaataccgct tgaatatggt attaaacatg
      241 atgcagatat tattattatg aatgacccag atgctgacag atttggaatg gcaataaaac
      301 atgatggtca ttttgtaaga ttagatggaa atcaaacagg accaatttta attgattgaa
      361 aattatcaaa tctaaaacgc ttaaatagca ttccaaaaaa tccggctcta tattcaagtt
      421 ttgtaacaag tgatttgggt gatagaatcg ctcatgaaaa atatggagtt aatattgtaa
      481 aaactttaac tggatttaaa tgaatgggta gagaaattgc taaagaagaa gataacggat
      541 taaattttgt
//
