LOCUS       sequence-98731-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sanchez-Rivera FJ, Papagiannakopoulos T, Romero R, Tammela T, Bauer 
            MR, Bhutkar A, Joshi NS, Subbaraj L, Bronson RT, Xue W, Jacks T
  TITLE     Rapid modelling of cooperating genetic events in cancer through 
            somatic genome editing.
  JOURNAL   Nature. 2014 Oct 22. doi: 10.1038/nature13906.
  PUBMED    25337879
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Oct 22. doi: 10.1038/nature13906."
COMMENT     Sequence Label: pSECC
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgctcaggcc gcctctctcg gccttggtca gattgtcgaa ctttctctgg gtaatcagct
       61 tggcgttcag cagctgccgc cagtagttct tcatcttctt cacgacctct tcggagggca
      121 cgttgtcgct cttgccccgg ttcttgtcgc ttctggtcag caccttgttg tcgatggagt
      181 cgtccttcag aaagctctga ggcacgatat ggtccacatc gtagtcggac agccggttga
      241 tgtccagttc ctggtccacg tacatatccc gcccattctg caggtagtac aggtacagct
      301 tctcgttctg cagctgggtg ttttccacgg ggtgttcttt caggatctgg ctgcccagct
      361 ctttgatgcc ctcttcgatc cgcttcattc tctcgcggct gttcttctgt cccttctggg
      421 tggtctggtt ctctctggcc atttcgatca cgatgttctc gggcttgtgc cggcccatca
      481 ctttcacgag ctcgtccacc accttcactg tctgcaggat gcccttctta atggcggggc
      541 tgccggccag attggcaatg tgctcgtgca ggctatcgcc ctggccggac acctgggctt
      601 tctggatgtc ctctttaaag gtcaggctgt cgtcgtggat cagctgcatg aagtttctgt
      661 tggcgaagcc gtcggacttc aggaaatcca ggattgtctt gccggactgc ttgtcccgga
      721 tgccgttgat cagcttccgg ctcagcctgc cccagccggt gtatctccgc cgcttcagct
      781 gcttcatcac tttgtcgtcg aacaggtggg cataggtttt cagccgttcc tcgatcatct
      841 ctctgtcctc
//
