LOCUS       sequence-98742-e         529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VP64 human MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-VP64 human MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..201)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggtcacctgc agcttggcgg tctgggtgcc ctcgtagggc ttgccctcgc cctcgccctc
       61 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaaggg
      121 cttgatgacg ttctcagtgc tatccatagg gccgggattc tcctccacgt caccgcatgt
      181 tagaagactt cctctgccct catgcatgag cacctggtat atcgggttgg ggttcgcacc
      241 cgcagggcac tgcggggcgg cgtccggtga ctgggtgggg tcgccgctgc tctctccctc
      301 gctgggggcg gcagcctctt gcctgcgcgg aggcgactca gaaggcacgt ccgccagcag
      361 gagggcgggc gccgcagggc tctcggtgga gatgcgctcc acgatgctgg acaggcagtc
      421 taggctcgac accgccgcac tcttcccggg cctgggttcg ctgggcgcct cgttgtagta
      481 ggcgccttcg tagcagttcc gccgccgggc gccgctcggg gggccgctg
//
