LOCUS       sequence-98781-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses two cyan fluorescent protein variants, a short 
            fluorescence lifetime CFP variant in the nucleus and a CFP variant 
            with a long lifetime (mTurquoise2) in the cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Joosen L, Hink MA, Gadella TW Jr, Goedhart J
  TITLE     Effect of fixation procedures on the fluorescence lifetimes of 
            Aequorea victoria derived fluorescent proteins.
  JOURNAL   J Microsc. 2014 Sep 1. doi: 10.1111/jmi.12168.
  PUBMED    25179491
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microsc. 
            2014 Sep 1. doi: 10.1111/jmi.12168."
COMMENT     Sequence Label: pH2B-SCFP1-2A-mTq2-NES
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..475
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      488..497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(494..496,497..499,500..958)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(539..560)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(800..819)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgccaccatg ccagagccag cgaagtctgc
      121 tcccgccccg aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa
      181 gaagcgcaag cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca
      241 ggtccaccct gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa
      301 cgacattttc gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc
      361 gaccatcacc tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc
      421 caagcacgcc gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc
      481 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      541 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      601 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      661 gccctggccc accctcgtga ccaccctgac ctggggcctg cagtgcttcg cccgctaccc
      721 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      781 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      841 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      901 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
//
