LOCUS       sequence-98864-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector with CaMKIIa promoter and upstream CMV promoter 
            expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors 
            changes in membrane voltage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Flytzanis NC, Bedbrook CN, Chiu H, Engqvist MK, Xiao C, Chan KY, 
            Sternberg PW, Arnold FH, Gradinaru V
  TITLE     Archaerhodopsin variants with enhanced voltage-sensitive 
            fluorescence in mammalian and Caenorhabditis elegans neurons.
  JOURNAL   Nat Commun. 2014 Sep 15;5:4894. doi: 10.1038/ncomms5894.
  PUBMED    25222271
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-15"; volume: "5:4894. doi"; pages: " 
            10.1038/ncomms5894"
COMMENT     Sequence Label: pLenti-CaMKIIa-Archer1-EGFP
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(129..150)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     478..497
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcttacacct cgttctcgta gcagaacttg tacagctcgt ccatgccgag agtgatcccg
      121 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      181 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      241 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      301 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      361 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      421 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      481 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      541 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa g
//
