LOCUS       sequence-98896-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H83L mutant of the mouse MSI1 RBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, 
            Ryder SP
  TITLE     Allosteric inhibition of a stem cell RNA-binding protein by an 
            intermediary metabolite.
  JOURNAL   Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848.
  PUBMED    24935936
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            Jun 16:e02848. doi: 10.7554/eLife.02848."
COMMENT     Sequence Label: pET-22HT-MSI1(7-192)H83L
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..74
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttgtttaact ttaagaagga gatatacata tgcatcatca tcatcatcac ggcgaaaacc
       61 tgtattttca gggcggatcc cagcccggcc tcgcctcccc ggactcgccg cacgacccct
      121 gcaagatgtt catcggagga ctcagttggc agaccacgca ggaagggctg cgcgaatact
      181 tcggccagtt cggggaggtg aaagagtgtc tggtgatgcg ggaccccctg accaaaagat
      241 ccaggggttt cggcttcgtc actttcatgg accaggcggg ggtggataaa gtgctggcgc
      301 aatctcggct ggagctcgac tccaaaacaa ttgaccccaa ggtggccttt cctcgaagag
      361 cacagcctaa gatggtcact cggacgaaga agatcttcgt gggggggctg tctgtgaaca
      421 ccacggtgga agatgtgaaa cactatttcg agcagttcgg aaaggtggat gatgccatgc
      481 tgatgttcga caaaaccacc aacaggcaca gagggtttgg atttgtcacg tttgagagcg
      541 aggacatcgt agagaaagtt tgtgagatcc acttccatga aatcca
//
