LOCUS       sequence-98910-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses constitutively active AMPKalpha2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     Birnbaum lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAMPK alpha2 delta312X
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             137..166
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcgagctctc tggctactga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc gccatggagc agaagcttat ctccgaggag gacctcggtg gcggcgagaa
      181 gcagaagcac gacgggcgtg tgaagatcgg acactacgtg ctgggggaca ccctgggcgt
      241 cggcaccttc ggcaaagtga agattggaga acatcaattg acaggccata aagtggcagt
      301 taagatctta aatagacaga agattcgcag tttagatgtt gttggaaaaa taaaacgaga
      361 aattcaaaat cttaaactct ttcgtcatcc tcatattatc aaactctacc aagtgatcag
      421 cactccaaca gactttttta tggtaatgga atatgtgtct ggaggtgaat tgttcgacta
      481 catctgtaaa cacgggaggg ttgaagaggt ggaagctcgc cggctcttcc agcagattct
      541 gtctgccgtg gactactgtc acaggcacat ggttgtccac agggacctga agccagagaa
      601 cgtgttgctg gacgcccaga tgaatgctaa gatagctgac ttcggactct ctaatatgat
      661 gtcagatggt gaatttctac gaactagctg tggatcgcca aattatgcag caccggaggt
      721 catctcagga aggctgtatg cgggtcctga ggttgatatc tggagctgtg gtgttatcct
      781 gtatgccctt ctctgtggca ccctcccgtt cgacgatgag cacgtgccta cgctctttaa
      841 gaaga
//
