LOCUS       sequence-98956-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enhance solubility and correct disulfide bond-formation of fused 
            protein expressed in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, Nguyen MT, Vu TT, Jeong 
            B, Kim SW, Choe H
  TITLE     Expression and purification of biologically active human FGF2 
            containing the b'a' domains of human PDI in Escherichia coli.
  JOURNAL   Appl Biochem Biotechnol. 2013 May;170(1):67-80. doi: 
            10.1007/s12010-013-0140-3. Epub 2013 Mar 8.
  PUBMED    23471584
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12010-013-0140-3"; journalName: "Appl Biochem Biotechnol";
            date: "2013-05"; volume: "170"; issue: "1"; pages: "67-80"
COMMENT     Sequence Label: pDEST-hPDIb′a′
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..74
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    900..924
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgaaa caccatcacc
       61 atcaccatca ccatgagctc attgaattta cagaacaaac ggcgccgaaa attttcggcg
      121 gtgagattaa aacacatatc ctgctgtttc tgccgaagag cgtttctgat tacgatggta
      181 aactgagtaa ttttaaaacc gccgcagaat ctttcaaagg taagattctg ttcattttca
      241 ttgatagcga ccacacggac aatcagcgta tcctggagtt ctttggtctg aagaaagagg
      301 aatgcccggc tgtgcgtctg attacgctgg aagaggaaat gacaaagtac aagccggaga
      361 gcgaggaact gactgcagaa cgtatcaccg aattttgtca tcgtttcctg gaggggaaga
      421 ttaagccgca tctgatgagc caggaactgc cggaggattg ggacaaacag ccagtgaaag
      481 ttctggtggg gaagaatttt gaagatgtgg ccttcgatga gaagaagaat gtgtttgtgg
      541 agttctacgc cccgtggtgt gggcactgta aacagctggc gccgatctgg gacaaactgg
      601 gcgaaacgta taaagatcac gaaaatattg tgatcgcgaa aatggattct accgcgaatg
      661 aagtagaagc ggtaaaagta cactcttttc cgacgctgaa attctttcca gcgagcgcgg
      721 atcgtactgt cattgattat aatggcgaac gtactctgga cggctttaag aaatttctgg
      781 aaagcggcgg ccaggatggc gcgggcgatg atgatgacct ggaagacctg gaagaagcgg
      841 aggaaccaga catggaggag gatgacgacc agaaagcggt cggtaccgga tcttacatca
      901 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga t
//
