LOCUS       sequence-99101-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) driven by MET17 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB649
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(59..75)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(59..75)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(76..425)
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     complement(476..496)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(476..494)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(515..531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(515..531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(528..550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    539..555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(563..569,570..587,588..593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    608..629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(746..763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt gacgtcttcc atgttaatta acccggggga
       61 tccactagtt ctagagtatg gatgggggta atagaattgt atctatgtat ctgacgaccc
      121 tgtattacac gaaggaaata gtagatgaaa agacaagaga gcaagaaaaa ggaaaaactt
      181 cctttctaac agacgcttta cttaacctta tatatatctt atttttttca tcgagcgtgt
      241 tcaattggac aaggtgccac cttttcgaca cttcggttat tatgttacac agttttcatg
      301 aggatggcgc cttgactaac ttaattagtc atttgccaac caccacagtt ccccaatatc
      361 gacagcttca cgtgccattt gccattttgc gatcatgtga cctcaagaga aaaagcaaaa
      421 aataatgaga gctaagggat tcgaaccctt gcatccggag ctccagcttt tgttcccttt
      481 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      541 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      601 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      661 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      721 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      781 tgcggcgagc ggtatcagct cactcaaggc ggtaatacgg ttatccacag aatcagggga
      841 taacgcag
//
