LOCUS       sequence-99235-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Sanchez-Rivera FJ, Papagiannakopoulos T, Romero R, Tammela T, Bauer 
            MR, Bhutkar A, Joshi NS, Subbaraj L, Bronson RT, Xue W, Jacks T
  TITLE     Rapid modelling of cooperating genetic events in cancer through 
            somatic genome editing.
  JOURNAL   Nature. 2014 Oct 22. doi: 10.1038/nature13906.
  PUBMED    25337879
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Oct 22. doi: 10.1038/nature13906."
COMMENT     Sequence Label: pSECC
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             116..139
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             146..166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
       61 aggtgtcgtg acgcgggacc cgacattagc gctacagctt aagcgggcca ccatggatta
      121 caaagacgat gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt
      181 cccagcagcc gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg
      241 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac
      301 cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac
      361 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg
      421 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt
      481 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat
      541 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct
      601 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct
      661 ggcccacatg atcaagttcc
//
