LOCUS       sequence-99240-e         581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT human MyoD and dsRedExpress2 in response to 
            doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-WT human MyoD-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             388..441
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 agcggcgact ccgacgcgtc cagcccgcgc tccaactgct ccgacggcat gatggactac
       61 agcggccccc cgagcggcgc ccggcggcgg aactgctacg aaggcgccta ctacaacgag
      121 gcgcccagcg aacccaggcc cgggaagagt gcggcggtgt cgagcctaga ctgcctgtcc
      181 agcatcgtgg agcgcatctc caccgagagc cctgcggcgc ccgccctcct gctggcggac
      241 gtgccttctg agtcgcctcc gcgcaggcaa gaggctgccg cccccagcga gggagagagc
      301 agcggcgacc ccacccagtc accggacgcc gccccgcagt gccctgcggg tgcgaacccc
      361 aacccgatat accaggtgct catgcatgag ggcagaggaa gtcttctaac atgcggtgac
      421 gtggaggaga atcccggccc tatggatagc actgagaacg tcatcaagcc cttcatgcgc
      481 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      541 gagggcaagc cctacgaggg cacccagacc gccaagctgc a
//
