LOCUS       sequence-99243-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hSK
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..44
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    133..166
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
       61 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ctcaattccg atcatattca
      121 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tctgaagagg
      181 agtttacgtc cagccaagct taggatcaat tctcatgttt gacagcttat catcgataag
      241 ctgatcctca caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg
      301 tgaccttgaa gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa
      361 gggggacgag gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca
      421 gcgggagttt tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg
      481 ggaattttca gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc
      541 tgttggaggg ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg
      601 tctattgatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa
      661 atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc
      721 aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg
      781 gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga
      841 gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag
      901 gagcagga
//
