LOCUS       sequence-99245-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hSK
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(221..254)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt gctcccattc ttaggtgaat
       61 ttaaggaggc caggctaaag ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa
      121 tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa catgggtatg
      181 cccaattgag atccgctagt gaacctcttc gagggaccta ataacttcgt atagcataca
      241 ttatacgaag ttatattaag ggttccggca attgccccat gttgggagga cgaaaatggt
      301 gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg gggatttatt
      361 ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct aacaagttac
      421 atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg tcatctccgt
      481 catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc aaatctactc
      541 catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc tttgtcataa
      601 caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt aaaaagacca
      661 tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt ccaggggcca
      721 ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca agggcagttc
      781 ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca ccggcgaccc
      841 aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct cttaaacctt
      901 ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt ccaaaccacc
      961 ctcctttttt gcgcctgcct ccatcaccct gaccccgggg tccagtgctt gggccttctc
     1021 ctgggtcatc tgcggggccc tgctctatcg ctcc
//
