LOCUS       sequence-99253-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hSK
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(494..513)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(575..597)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gcccgcgccc aggccggcgc ccaccccgac cccgctcgcc atgctattgc cgccgggggc
       61 cagcagcccg ccgggcagcg tgtacttatc cttcttcatg agcgtcttgg ttttccgccg
      121 gggccggtat ttataatccg ggtgctcctt catgtgcagc gctcgcagcc gcttagcctc
      181 gtcgatgaac ggccgcttct ccgtctccga caaaagtttc cactcggcgc ccaggcgctt
      241 gctgatctcc gagttgtgca tcttggggtt ctcctgggcc atcttgcgcc gctgcccgcg
      301 ggaccacacc atgaaggcat tcatgggccg cttgacgcgg tccgggctgt ttttctggtt
      361 gccgccggcc gccgccgcgg tggagttgcc gccgccgccc cccgaagttt gctgcgggcc
      421 cggcggcttc agctccgtct ccatcatgtt gtacatggtg aagggcgaat tctttgccaa
      481 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
      541 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
      601 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
      661 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
      721 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt
      781 tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata
      841 aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc
      901 gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccgg
//
