LOCUS       sequence-99255-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hSK
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
       61 ttattgtgct gtctcatcat tttggcaaag aattcgccct tcaccatgta caacatgatg
      121 gagacggagc tgaagccgcc gggcccgcag caaacttcgg ggggcggcgg cggcaactcc
      181 accgcggcgg cggccggcgg caaccagaaa aacagcccgg accgcgtcaa gcggcccatg
      241 aatgccttca tggtgtggtc ccgcgggcag cggcgcaaga tggcccagga gaaccccaag
      301 atgcacaact cggagatcag caagcgcctg ggcgccgagt ggaaactttt gtcggagacg
      361 gagaagcggc cgttcatcga cgaggctaag cggctgcgag cgctgcacat gaaggagcac
      421 ccggattata aataccggcc ccggcggaaa accaagacgc tcatgaagaa ggataagtac
      481 acgctgcccg gcgggctgct ggcccccggc ggcaatagca tggcgagcgg ggtcggggtg
      541 ggcgccggcc tgggcgcggg cgtgaaccag cgcatggaca gttacgcgca catgaacggc
      601 tggagcaacg gcagctacag catgatgcag gaccagctgg gctacccgca gcacccgggc
      661 ctcaatgcgc acggcgcagc gcagatgcag cccatgcacc gctacgacgt gagcgccctg
      721 cagtacaact ccatgaccag ctcgcagacc tacatgaacg gctcgcccac ctacagcatg
      781 tcctactcgc agcagggcac ccctggcatg gctcttggct ccatgggttc ggtggtcaag
      841 tccgaggcca gctccagccc ccctgtggtt acctcttcct cccactccag ggcgccctgc
      901 caggccgggg acctccggga catgatcagc atgtatctcc ccggcgccga ggtgccggaa
      961 cccgccgccc ccagcagact tcacatgtcc cagcactacc agagcggccc ggtgcccggc
     1021 acggccatta acggcacact gcccctctca cacatgggg
//
