LOCUS       sequence-99284-e         535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human OCT3/4 and shRNA 
            against p53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hOCT3/4-shp53-F
FEATURES             Location/Qualifiers
     source          1..535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..239
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     301..320
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
       61 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
      121 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
      181 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
      241 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
      301 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcgcc cttcaccatg
      361 gcgggacacc tggcttcgga tttcgccttc tcgccccctc caggtggtgg aggtgatggg
      421 ccaggggggc cggagccggg ctgggttgat cctcggacct ggctaagctt ccaaggccct
      481 cctggagggc caggaatcgg gccgggggtt gggccaggct ctgaggtgtg gggga
//
