LOCUS       sequence-99286-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human OCT3/4 and shRNA 
            against p53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hOCT3/4-shp53-F
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     215..232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(383..696)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(444..464)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
       61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      181 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      241 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcgg
      301 atcccccggg ctgcaggaat tccaaaaaag actccagtgg taatctactc tcttgaagta
      361 gattaccact ggagtcggcc caaacaaggc ttttctccaa gggatattta tagtctcaaa
      421 acacacaatt actttacagt tagggtgagt ttccttttgt gctgtttttt aaaataataa
      481 tttagtattt gtatctctta tagaaatcca agcctatcat gtaaaatgta gctagtatta
      541 aaaagaacag attatctgtc ttttatcgca cattaagcct ctatagttac taggaaatat
      601 tatatgcaaa ttaaccgggg caggggagta gccgagcttc tcccacaagt ctgtgcgagg
      661 gggccggcgc gggcctagag atggcggcgt cggatccgct agcgtcgact ctagaggatc
      721 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
      781 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
      841 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
      901 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
      961 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     1021 ctggg
//
