LOCUS       sequence-99346-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of firefly luciferase and tandem Tomato red 
            fluorescent protein in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Patel MR, Chang YF, Chen IY, Bachmann MH, Yan X, Contag CH, Gambhir 
            SS
  TITLE     Longitudinal, noninvasive imaging of T-cell effector function and 
            proliferation in living subjects.
  JOURNAL   Cancer Res. 2010 Dec 15;70(24):10141-9.
  PUBMED    21159636
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2010-12-15"; volume: "70(24)"; pages: "10141-9"
COMMENT     Sequence Label: pcDNA3.1(+)/Luc2=tdT
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc accaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
      181 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
      241 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
      301 gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
      361 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
      421 cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
      481 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa
      541 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
      601 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
      661 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
      721 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
      781 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
      841 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
      901 cttgatctgc ggcttt
//
