LOCUS       sequence-99348-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of firefly luciferase and tandem Tomato red 
            fluorescent protein in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Patel MR, Chang YF, Chen IY, Bachmann MH, Yan X, Contag CH, Gambhir 
            SS
  TITLE     Longitudinal, noninvasive imaging of T-cell effector function and 
            proliferation in living subjects.
  JOURNAL   Cancer Res. 2010 Dec 15;70(24):10141-9.
  PUBMED    21159636
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2010-12-15"; volume: "70(24)"; pages: "10141-9"
COMMENT     Sequence Label: pcDNA3.1(+)/Luc2=tdT
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(131..154)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(329..348)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     598..616
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctagactcga gcggccgcca ctgtgctgga tatctgcaga attcttactt gtacagctcg
       61 tccatgccgt acaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg
      121 gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc
      181 agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta gtggccgccg
      241 tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
      301 cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg
      361 aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc
      421 tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      481 tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg
      541 gagccgtaca tgaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg
      601 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
      661 atctcgaact cgtggccgtt catggagccc tcc
//
