LOCUS       sequence-99363-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-wt
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(343..370)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc ttatcatcga
       61 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
      121 caagccgtca attgtctgat tcgttaccaa cagacattgc cgtcactgcg tcttttactg
      181 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
      241 gaccaaagcc atgacaaaaa cgcgtaattt caacacgagc caaaataata aaaaagcagg
      301 attaataatc tggcttttta tattctctct ctagtatata aacgcagaaa ggcccacccg
      361 aaggtgagcc agtgtgatcc aagccagctc gatatccgtc ggcttgaacg aattgttaga
      421 cattacttgt agacgtgccc tacgcgcttg ttgagttgtt cctcaacgtt tttcttgaag
      481 tgcttagctt caaggtcacg gatacgaccg aagcgagcct cgtcctcaat ggcccgaccg
      541 attgcgcttg ctacagcctg aacggttgta ttgtcagcac tggttaggca agccagagtg
      601 gtcttaatgg tgatgtacgc tacggcttcc ggcttgattt cttgcaggaa ctggaaggct
      661 gtcgggcgct tgccgcgctt agctttcact tcctcaaacc agtcgttgat gcgtgcaatc
      721 atcttaggga gtagggtagt gatgagaggc ttggcggcag cgttatccgc aacctcacca
      781 gctttaagtt gacgctcaaa catcttgcgg aagcgtgctt cacccatctc gtaagactca
      841 tgctcaaggg ccaactgttc gcgagctaaa cgctcaccgt aatggtcagc cagagtgttg
      901 aacggg
//
