LOCUS       sequence-99381-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal fragment of split T7 RNAP split a position 
            179. Insert is driven by PBAD. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-N
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     898..917
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
       61 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      121 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      181 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      241 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      301 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      361 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      421 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      481 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      541 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      601 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
      661 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
      721 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
      781 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
      841 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
      901 ccatagcatt tttatccata agattagcgg
//
