LOCUS       sequence-99395-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enhance solubility and correct disulfide bond-formation of fused 
            protein expressed in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, Nguyen MT, Vu TT, Jeong 
            B, Kim SW, Choe H
  TITLE     Expression and purification of biologically active human FGF2 
            containing the b'a' domains of human PDI in Escherichia coli.
  JOURNAL   Appl Biochem Biotechnol. 2013 May;170(1):67-80. doi: 
            10.1007/s12010-013-0140-3. Epub 2013 Mar 8.
  PUBMED    23471584
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12010-013-0140-3"; journalName: "Appl Biochem Biotechnol";
            date: "2013-05"; volume: "170"; issue: "1"; pages: "67-80"
COMMENT     Sequence Label: pDEST-hPDI
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..271)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc agcttttttg tacaaacttg tgatgtaaga tccggtaccg accgctttct
      301 ggtcgtcatc ctcctccatg tctggttcct ccgcttcttc caggtcttcc aggtcatcat
      361 catcgcccgc gccatcctgg ccgccgcttt ccagaaattt cttaaagccg tccagagtac
      421 gttcgccatt ataatcaatg acagtacgat ccgcgctcgc tggaaagaat ttcagcgtcg
      481 gaaaagagtg tacttttacc gcttctactt cattcgcggt agaatccatt ttcgcgatca
      541 caatattttc gtgatcttta tacgtttcgc ccagtttgtc ccagatcggc gccagctgtt
      601 tacagtgccc acaccacggg gcgtagaact ccacaaacac attcttcttc tcatcgaagg
      661 ccacatcttc aaaattcttc cccaccagaa ctttcactgg ctgtttgtcc caatcctccg
      721 gcagttcctg gctcatcaga tgcggcttaa tcttcccc
//
