LOCUS       sequence-99412-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the C-terminal fragment of split T7 RNAP split a position 
            179. Insert is driven by the arabinose inducible promoter PBAD. 
            Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-C
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     798..817
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
       61 ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat
      121 atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
      181 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
      241 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
      301 cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga
      361 aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc
      421 gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc
      481 catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc ctttgattcc
      541 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
      601 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      661 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      721 aaatatagtt ctttcctgta cataaccttc gggcactcga gtccacattg attatttgca
      781 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
//
