LOCUS       sequence-99432-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A modified pET28 driving expression of GFP, biobrick part Bba_E0040.
            No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' 
            between -11 and -8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pET:GACG:GFP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     407..435
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(600..619)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtctgccatg atgtatacat tgtgtgagtt atagttgtat tccaatttgt gtccaagaat
       61 gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa gggtatcacc
      121 ttcaaacttg acttcagcac gtgtcttgta gttcccgtca tctttgaaaa atatagttct
      181 ttcctgtaca taaccttcgg gcatggcact cttgaaaaag tcatgctgtt tcatatgatc
      241 tgggtatctc gcaaagcatt gaacaccata accgaaagta gtgacaagtg ttggccatgg
      301 aacaggtagt tttccagtag tgcaaataaa tttaagggta agttttccgt atgttgcatc
      361 accttcaccc tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac
      421 aagaattggg acaactccag tgaaaagttc ttctccttta cgcatgacgt cggtatatct
      481 ccttcttaaa gttaaaccct atagtgcgtc gtattaattt cgcgggatcg agatctcgat
      541 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      601 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
      661 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
      721 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
      781 cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc
      841 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc a
//
