LOCUS       sequence-99482-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy Esp3I-ccdB A-B
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             455..760
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     639..658
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
       61 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatctagag
      121 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      181 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
      241 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
      301 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
      361 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
      421 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
      481 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
      541 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
      601 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
      661 cagtgtgccg gtttccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
      721 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
      781 cagccagtct gcaggtcgac ggtctcttct gtgagacgtt cgaatgcatc tagatatcgg
      841 atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggcg
//
