LOCUS       sequence-99485-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C N-NLS KOZ c2
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     259..277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(315..337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     437..456
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     650..672
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     664..681
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     665..681
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(742..762)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt
       61 gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg
      121 catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt
      181 tcaagccgag atcggcttcc cggccgcgga gttgttcggt aaattgtcac aacgccgctc
      241 atgacattaa cctataaaaa taggcgtatc acgaggccct tttgtctcgc gcgtttcggt
      301 gatgacggtg aaaacctctg acacatgcag ctcccggaga aggtcacagc ttgtctgtaa
      361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      421 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      481 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
      541 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      601 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      661 cgttgtaaaa cgacggccag tgaattcgag ctcggtacct cgtaggtctc aggtggcggc
      721 cgcactcgag tcgactcctc caacctttct cttcttctta ggctgcagca ttgttcagat
      781 gagaccatcg aatgcatcta gatatcggat cccgggcccg tcgactgcag aggcctgcat
      841 gcaagctggc g
//
