LOCUS       sequence-99518-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains full length native galactose promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: PGAL
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        66..507
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    66..183
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     478..501
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 aagctggagc tccgggatcc ttggcgcgcc ccttaattaa cggaagcttc ctgtttaaac
       61 tagtacggat tagaagccgc cgagcgggcg acagccctcc gacggaagac tctcctccgt
      121 gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg ccgcactgct
      181 ccgaacaata aagattctac aatactagct tttatggtta tgaagaggaa aaattggcag
      241 taacctggcc ccacaaacct tcaaattaac gaatcaaatt aacaaccata ggatgataat
      301 gcgattagtt ttttagcctt atttctgggg taattaatca gcgaagcgat gatttttgat
      361 ctattaacag atatataaat ggaaaagctg cataaccact ttaactaata ctttcaacat
      421 tttcagtttg tattacttct tattcaaatg tcataaaagt atcaacaaaa aattgttaat
      481 atacctctat actttaacgt caaggagaaa aaaccccgga ttctagaaaa atgtctaaag
      541 gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt gatgttaatg
      601 gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt aaattgacct
      661 taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta gtcactactt
      721 taggttatgg tttgatgtgt tttgctagat acccagatca tatgaaa
//
