LOCUS       sequence-99556-e       15676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""CoinFLP-Gal4"" - Expresses Gal4 from actin promoter downsteam of 
            transcriptional stop. Recombination by FLP excises FRT-FRT pair to 
            express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15676)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 15676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..15676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4850..7304
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5c promoter"
     protein_bind    9128..9161
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    9537..9570
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    9591..9624
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    9665..9698
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             9804..12449
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     intron          12539..12604
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12734..12754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13026..13160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13063..13082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13117..13136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    13200..13269
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(13488..13506)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13527..13543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13527..13543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13540..13562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13551..13567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13575..13581,13582..13599,13600..13605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13620..13641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13758..13775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13929..14517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14009..14028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14688..15479,15480..15548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15311..15330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15549..15653)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atctcgctgc
     4861 ctgttatgtg gcccacaaac caagacacgt tttatggcca ttaaagctgg ctgatcgtcg
     4921 ccaaacacca aatacataat gaatatgtac acattcgaga aagaagcgat caaagaagcg
     4981 tcttcgggcg gagtaggaga atgcggagga gaaggagaac gagctgatct agtatctctc
     5041 cacaatccaa tgccaactga ccaactggcc atattcggag caatttgaag ccaatttcca
     5101 tcgcctggcg atcgctccat tcttggctat atgtttttca ccgttacccg gggccatttt
     5161 caaagactcg tcggcaagat aagattgtgt cactcgctgt ctctcttcat ttgtcgaaga
     5221 atgctgagga atttcgcgat gacgtcggcg agtattttga agaatgagaa taatttgtat
     5281 ttatacgaaa atcagttagt ggaattttct acaaaaacat gttatctata gataattttg
     5341 ttgcaaaata tgttgactat gacaaagatt gtatgtatat acctttaatg tattctcatt
     5401 ttcttatgta tttataatgg caatgatgat actgatgata ttttaagatg atgccagacc
     5461 acaggctgat ttctgcgtct tttgccgaac gcagtgcatg tgcggttgtt gttttttgga
     5521 atagtttcaa ttttcggact gtccgctttg atttcagttt cttggcttat tcaaaaagca
     5581 aagtaaagcc aaaaaagcga gatggcaata ccaaatgcgg caaaacggta gtggaaggaa
     5641 aggggtgcgg ggcagcggaa ggaagggtgg ggcggggcgt ggcggggtct gtggctgggc
     5701 gcgacgtcac cgacgttgga gccactcctt tgaccatgtg tgcgtgtgtg tattattcgt
     5761 gtctcgccac tcgccggttg tttttttctt tttatctcgc tctctctagc gccatctcgt
     5821 acgcatgctc aacgcaccgc atgttgccgt gtcctttatg cgtcattttg gctcgaaata
     5881 ggcaattatt taaacaaaga ttagtcaacg aaaacgctaa aataaataag tctacaatat
     5941 ggttacttat tgccatgtgt gtgcagccaa cgatagcaac aaaagcaaca acacagtggc
     6001 tttccctctt tcactttttg tttgcaagcg cgtgcgagca agacggcacg accggcaaac
     6061 gcaattacgc tgacaaagag cagacgaagt tttggccgaa aaacatcaag gcgcctgata
     6121 cgaatgcatt tgcaataaca attgcgatat ttaatattgt ttatgaagct gtttgacttc
     6181 aaaacacaca aaaaaaaaaa taaaacaaat tatttgaaag agaattagga atcggacagc
     6241 ttatcgttac gggctaacag cacaccgaga cgaaatagct tacctgacgt cacagcctct
     6301 ggaagaactg ccgccaagca gacgatgcag aggacgacac atagagtagc ggagtaggcc
     6361 agcgtagtac gcatgtgctt gtgtgtgagg cgtctctctc ttcgtctcct gtttgcgcaa
     6421 acgcatagac tgcactgaga aaatcgatta cctatttttt atgaatgaat atttgcacta
     6481 ttactattca aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg
     6541 agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt
     6601 atagaaatgc ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt
     6661 taaaaccggc ttaagttagg tgtgttttct acgactagtg atgcccctac tagaagatgt
     6721 gtgttgcaca aatgtccctg aataaccaat ttgaagtgca gatagcagta aacgtaagct
     6781 aatatgaata ttatttaact gtaatgtttt aatatcgctg gacattacta ataaacccac
     6841 tataaacaca tgtacatatg tatgttttgg catacaatga gtagttgggg aaaaaatgtg
     6901 taaaagcacc gtgaccatca cagcataaag ataaccagct gaagtatcga atatgagtaa
     6961 cccccaaatt gaatcacatg ccgcaactga taggacccat ggaagtacac tcttcatggc
     7021 gatatacaag acacacacaa gcacgaacac ccagttgcgg aggaaattct ccgtaaatga
     7081 aaacccaatc ggcgaacaat tcatacccat atatggtaaa agttttgaac gcgacttgag
     7141 agcggagagc attgcggctg ataaggtttt agcgctaagc gggctttata aaacgggctg
     7201 cgggaccagt tttcatatca ctaccgtttg agttcttgtg ctgtgtggat actcctcccg
     7261 acacaaagcc gctccatcag ccagcagtcg tctaatccag agacaccaaa ccgaaagact
     7321 taatttatat ttatttaatt aattttaata aaacacacca aatgtaagta gctttcccct
     7381 tcccaacaac aaaacaccat cgaaccactc ccaccaagaa aaagcaataa tcgagaaaag
     7441 ccgcggaaaa tgtgtgattt tttttgtaaa caaaacattt ttttatgtgc cagtgctgaa
     7501 agtgatcaaa aaatactagc cacgagctaa agagttattg tattgaccaa aactccaaaa
     7561 atacccaagt ttggccctaa attgtcaatc aaaataccaa taggtcgaaa gacatcaaaa
     7621 ttaacaaaac cagggtttca aataccataa ctcaagaatc aggattacaa ctgcagattt
     7681 caggatatat acatacaaat tatacgaaat tataaaaacc aaagcaattc aatagcccca
     7741 actcaaatgt taggatctaa tatagtgttt aaagccaagc tcgctgatgt gggcgtgtca
     7801 cgatttcacc caaagatatg ccaaattacg aattgcaaat caattcgcca acactttctt
     7861 tttttcccac gccctaaaac accagatcat ccataaatgt acatacatac agtatatgca
     7921 tattataatc tgtaaaacta gatcaggttc ttgaaaatag tgacgtaggc agccgttttg
     7981 gctgaagcag aaatttttgc cggtttttca aagttgtagt tgcaaaaatg gagaaaacct
     8041 tcgagcattc gttcatatac acacactcac gcgcaaaata acgagagaga gtgtatgtgt
     8101 gtgtgagaga gcgaaagcca gacgacggtt tgcttttcgc ctcgaaacat gaccatatat
     8161 ggtcacaaaa cttggccgcc gcaattcaac acaccagcgc tctccttcgc acccatagcg
     8221 accatggcgc ggagcgagcg agatggcgag agcgagcgac gcctatggcg acgtcgacgc
     8281 aggcagcgat tgaaaaacgc agttaactgg cattcaacat tcaccagcca ctttcagtcg
     8341 gtttattcca gtcattcctt tcaaaccgtg cggtcgctta gctcagcctc gccacttgcg
     8401 tttacagtag ttttcacgcc ttgaatttgt taaatcgaac aaaaaggtaa agtttaacta
     8461 gctttgaaaa gtttcgtggc tcttaattgt taaattttct agagtgcgtt tagtgttttt
     8521 tttttttttt attttgtaat gttaatttcg ggttccaatt cgagttttag gcagccgcca
     8581 ttttaagggc gcatacacac aggcaactgt gctctctttg cggctttctt ttgcaccggc
     8641 attcgttaag ctgtcgtcta gaagcttctc ccctcccttt tcggcatatt cgtattgtgg
     8701 ttttaatttt tcggggcggg gcttctattt tgtaactgtt cttttaattt cttattacaa
     8761 ttcgatcgca agtgaaaatc agttttcaat cggaaaagta tttttttatg aaattttttt
     8821 ttgtccaaga ttaaaatttt gtactaaaaa aacgtacatt gcattgagtg atttttaatt
     8881 gtacacgaaa aacaagttag tttgttatga caattgtact ttggtagacc agcgcagtcc
     8941 aaggaaacca cgcaaattct cagttttttt tttgccattt ctacattacc aaataaggta
     9001 accaaaaact aatgggaaat ccgcattctt tccattgcag cttacaggat cgatccccgg
     9061 gtacggctag tggatcagct tgcatgcctg caggtcccct cgaggggact ctagctagag
     9121 gatcccggaa gttcctattc tctagaaagt ataggaactt cgaattgact aaagccaaat
     9181 agaaaattat tcagttcctg gcttaagttt ttaaaagtga tattatttat ttggttgtaa
     9241 ccaaccaaaa gaatgtaaat aactaataca taattatgtt agttttaagt tagcaacaaa
     9301 ttgattttag ctatattagc tacttggtta ataaatagaa tatatttatt taaagataat
     9361 tgcgttttta ttgtcaggga gtgagtttgc ttaaaaactc gtttagatcc actagttcta
     9421 gttgcggccg gccagcggcc gctgggttca ggcatagagg agttgacgac tacttccaag
     9481 gcagtacgtg acgtaacggc ccttaaagtg atcagtcctt attgctttgt ctgccggaag
     9541 ttcctattct tcaaatagta taggaacttc gtgtgaggga gcgtatttgg gaagttccta
     9601 ttctctagaa agtataggaa cttccccgct taccaccata catccaaatc actacggctc
     9661 gcttgaagtt cctattcttc aaatagtata ggaacttcga tggtgtagtg ttgcggtgga
     9721 attcagatct taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagcccaa
     9781 gcttgaagca agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata
     9841 tttgccgact taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga
     9901 agaacaactg ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac
     9961 atctgacaga agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc
    10021 ctcgagaaga ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt
    10081 taacaggatt atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt
    10141 cagtggagac tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat
    10201 cggaagagag tagtaacaaa ggtcaaagac agttgactgt atcgattgac tcggcagctc
    10261 atcatgataa ctccacaatt ccgttggatt ttatgcccag ggatgctctt catggatttg
    10321 attggtctga agaggatgac atgtcggatg gcttgccctt cctgaaaacg gaccccaaca
    10381 ataatgggtt ctttggcgac ggttctctct tatgtattct tcgatctatt ggctttaaac
    10441 cggaaaatta cacgaactct aacgttaaca ggctcccgac catgattacg gatagataca
    10501 cgttggcttc tagatccaca acatcccgtt tacttcaaag ttatctcaat aattttcacc
    10561 cctactgccc tatcgtgcac tcaccgacgc taatgatgtt gtataataac cagattgaaa
    10621 tcgcgtcgaa ggatcaatgg caaatccttt ttaactgcat attagccatt ggagcctggt
    10681 gtatagaggg ggaatctact gatatagatg ttttttacta tcaaaatgct aaatctcatt
    10741 tgacgagcaa ggtcttcgag tcaggttcca taattttggt gacagcccta catcttctgt
    10801 cgcgatatac acagtggagg cagaaaacaa atactagcta taattttcac agcttttcca
    10861 taagaatggc catatcattg ggcttgaata gggacctccc ctcgtccttc agtgatagca
    10921 gcattctgga acaaagacgc cgaatttggt ggtctgtcta ctcttgggag atccaattgt
    10981 ccctgcttta tggtcgatcc atccagcttt ctcagaatac aatctccttc ccttcttctg
    11041 tcgacgatgt gcagcgtacc acaacaggtc ccaccatata tcatggcatc attgaaacag
    11101 caaggctctt acaagttttc acaaaaatct atgaactaga caaaacagta actgcagaaa
    11161 aaagtcctat atgtgcaaaa aaatgcttga tgatttgtaa tgagattgag gaggtttcga
    11221 gacaggcacc aaagttttta caaatggata tttccaccac cgctctaacc aatttgttga
    11281 aggaacaccc ttggctatcc tttacaagat tcgaactgaa gtggaaacag ttgtctctta
    11341 tcatttatgt attaagagat tttttcacta attttaccca gaaaaagtca caactagaac
    11401 aggatcaaaa tgatcatcaa agttatgaag ttaaacgatg ctccatcatg ttaagcgatg
    11461 cagcacaaag aactgttatg tctgtaagta gctatatgga caatcataat gtcaccccat
    11521 attttgcctg gaattgttct tattacttgt tcaatgcagt cctagtaccc ataaagactc
    11581 tactctcaaa ctcaaaatcg aatgctgaga ataacgagac cgcacaatta ttacaacaaa
    11641 ttaacactgt tctgatgcta ttaaaaaaac tggccacttt taaaatccag acttgtgaaa
    11701 aatacattca agtactggaa gaggtatgtg cgccgtttct gttatcacag tgtgcaatcc
    11761 cattaccgca tatcagttat aacaatagta atggtagcgc cattaaaaat attgtcggtt
    11821 ctgcaactat cgcccaatac cctactcttc cggaggaaaa tgtcaacaat atcagtgtta
    11881 aatatgtttc tcctggctca gtagggcctt cacctgtgcc attgaaatca ggagcaagtt
    11941 tcagtgatct agtcaagctg ttatctaacc gtccaccctc tcgtaactct ccagtgacaa
    12001 taccaagaag cacaccttcg catcgctcag tcacgccttt tctagggcaa cagcaacagc
    12061 tgcaatcatt agtgccactg accccgtctg ctttgtttgg tggcgccaat tttaatcaaa
    12121 gtgggaatat tgctgatagc tcattgtcct tcactttcac taacagtagc aacggtccga
    12181 acctcataac aactcaaaca aattctcaag cgctttcaca accaattgcc tcctctaacg
    12241 ttcatgataa cttcatgaat aatgaaatca cggctagtaa aattgatgat ggtaataatt
    12301 caaaaccact gtcacctggt tggacggacc aaactgcgta taacgcgttt ggaatcacta
    12361 cagggatgtt taataccact acaatggatg atgtatataa ctatctattc gatgatgaag
    12421 ataccccacc aaacccaaaa aaagagtaag gtacctctag aggatctttg tgaaggaacc
    12481 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
    12541 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
    12601 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
    12661 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
    12721 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
    12781 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
    12841 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat
    12901 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
    12961 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    13021 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    13081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    13141 tatcttatca tgtctggatc cactagagat ccactagtgt cgacgatgta ggtcacggtc
    13201 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
    13261 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
    13321 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
    13381 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
    13441 acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct ttagtgaggg
    13501 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    13561 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    13621 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    13681 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    13741 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    13801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    13861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    13921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    13981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    14041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    14101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    14161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    14221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    14281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    14341 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    14401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    14461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    14521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    14581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    14641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    14701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    14761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    14821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    14881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    14941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    15001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    15061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    15121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    15181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    15241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    15301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    15361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    15421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    15481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    15541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    15601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    15661 ccccgaaaag tgccac
//
