LOCUS       sequence-99581-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC recombineering generated construct, used as a reporter for Oct4 
            expression (containes both DE and PE elements) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: mouse Oct4-GFP GOF18 transgenic reporter
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..20)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4..20)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        31..49
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggggatccac tagttctaga gcggccgcgg aattaaccct cactaaaggg aacgaattcg
       61 gatcatgtta attctgacta caagtctgtt cctgttctcg tcacctgtct tatgtctgtc
      121 tcattatcac cagggacttt ggtcaataat gtccgacttc caagaggcca cagactggag
      181 ttgagtgatg gtgaccttct gacctttggc ccccaaggtc aagcaggaac cagctcctcc
      241 tcagagttct acttcatgtt tcaacaagtc cgggttaaac ctcaggactt tgcagccata
      301 accgtccctc ggtctaaggg agaagctggg ggcggtttcc aacctatgct gcccccccaa
      361 ggggcacctc agaggccact cagcactctc tcctctgccc ccaaggccac actgattctc
      421 aattccatcg gcagcctcag caaactccag gcccagcctc tcaccttctc ccgtggtggt
      481 ggcaggccac agggcctggc tattccctct cagcatgggg aagcgcaagt ttcgcctgct
      541 ccacccacaa gaaaccggag gaaatcagct cataaagtgt tggcagagct agatgatgag
      601 gtctccccag gccccctgtc cgtcctgacg gagcccagga agaggctccg ggtggagaaa
      661 gctgctctga tagccagtgg gtaagtgggg tcacctgtca gcaccttgaa ttctagatct
      721 ttgaacagcc tcagactgag gtcttagaag gcagggaagg ggggtgcacg ggcacagcag
      781 acacccaggt tagctttcac ccataaatgc atgacaagct actatgtggg
//
