LOCUS       sequence-99585-e        7637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Also referred to as p-Phi-RGNndm-1. Expresses Cas9, tracrRNA and a 
            guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, 
            kanamycin resistance cassette and f1 origin for packaging in M13 
            particles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pRC319
FEATURES             Location/Qualifiers
     source          1..7637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      11..97
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    164..295
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   296..331
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   362..397
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     rep_origin      complement(736..1324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(816..835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1412..1506)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1537..2331)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1648..1667)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2258..2277
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      2549..3004
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2636..2655)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2846..2867
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_RNA        complement(3155..3233)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        join(3398..3416,3417..3422,3423..3439,3440..3445,
                     3446..3471)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    3398..3416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3423..3441
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             3481..3492
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3507..3524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3525..7631
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3525..7628
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
       61 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagcaccc
      121 tgcagaacta tatgattttc cgcagtatat tttagatgaa gattatttct taataactaa
      181 aaatatggta taatactctt aataaatgca gtaatacagg ggcttttcaa gactgaagtc
      241 tagctgagac aaatagtgcg attacgaaat tttttagaca aaaatagtct acgaggtttt
      301 agagctatgc tgttttgaat ggtcccaaaa cgggcagtcg cttccaacgg tttgatcgtc
      361 agttttagag ctatgctgtt ttgaatggtc ccaaaacttc agcacactga gacttgttga
      421 gttccatgtt ttagagctat gctgttttga atggtctcca ttctctagat gatgattaag
      481 gatctatttt tttgggcggg gccgcccaaa aaaatggttc gcttgctgtc cataaaaccc
      541 cgggcagaag atcctgcagg gccttctcca tgcggccgcc ggtaggctag gcgttcggct
      601 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      661 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      721 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      781 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      841 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      901 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
      961 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1021 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1081 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1141 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     1201 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1261 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1321 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1381 ttaagggatt ttggtcatga ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     1441 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1501 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     1561 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     1621 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     1681 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     1741 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     1801 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     1861 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     1921 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     1981 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     2041 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     2101 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     2161 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     2221 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     2281 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     2341 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     2401 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     2461 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2521 cacgaggccc tttcgtcttc acctcgagac gcgccctgta gcggcgcatt aagcgcggcg
     2581 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2641 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2701 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2761 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2821 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2881 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2941 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3001 atttcctagg cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3061 tcacgaggcc ctttcgtctt caccctgtcg acgcgttttg aaagcgcttg tttttttggt
     3121 ttgcagtcag agtagaatag aagtatcaaa aaaagcaccg actcggtgcc actttttcaa
     3181 gttgataacg gactagcctt attttaactt gctatgctgt tttgaatggt tccaacaaga
     3241 ttattttata acttttataa caaataatca aggagaaatt caaagaaatt tatcagccat
     3301 aaaacaatac ttaatactat agaatgataa caaaataaac tactttttaa aagaattttg
     3361 tgttataatc tatttattat taagtattgg gatcgattcc ctatcagtga tagagattga
     3421 catccctatc agtgatagag atactgagca catcagcagg acgcactgac cgaattcatt
     3481 aaagaggaga aaggtgcggc cgcatgcatc accatcacca tcacatggat aagaaatact
     3541 caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata
     3601 aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa
     3661 atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac
     3721 ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt
     3781 tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt
     3841 tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag
     3901 ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta
     3961 ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg
     4021 gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta
     4081 tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag
     4141 tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca
     4201 ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat
     4261 tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc
     4321 tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat
     4381 atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc
     4441 taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg
     4501 atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa
     4561 agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg
     4621 gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta
     4681 ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg
     4741 acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac
     4801 aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt
     4861 ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga
     4921 ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg
     4981 cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa
     5041 aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa
     5101 aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga
     5161 aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag
     5221 aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata
     5281 gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt
     5341 ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat
     5401 ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata
     5461 aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat
     5521 tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag
     5581 atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag
     5641 aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa
     5701 atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg
     5761 aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg
     5821 aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag
     5881 aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat
     5941 tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc
     6001 aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt
     6061 tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta
     6121 aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac
     6181 ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg
     6241 gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc
     6301 aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa
     6361 atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact
     6421 tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg
     6481 atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat
     6541 cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg
     6601 agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct
     6661 tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta
     6721 atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag
     6781 tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct
     6841 ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact
     6901 gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg
     6961 ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga
     7021 tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag
     7081 gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt
     7141 tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc
     7201 tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga
     7261 agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt
     7321 tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca
     7381 atttagataa agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag
     7441 cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat
     7501 attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca
     7561 ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag
     7621 gaggtgactg actcgag
//
