LOCUS       sequence-99604-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct for lineage tracing and mosaic analysis in 
            zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Collins RT, Linker C, Lewis J
  TITLE     MAZe: a tool for mosaic analysis of gene function in zebrafish.
  JOURNAL   Nat Methods. 2010 Mar;7(3):219-23. doi: 10.1038/nmeth.1423. Epub 
            2010 Feb 7.
  PUBMED    20139970
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1423"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "219-23"
COMMENT     Sequence Label: pMAZe
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..129
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             213..233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    405..526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(442..461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     496..515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(670..691)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
       61 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
      121 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
      181 tgttcctgta cggcatggac gagctgtaca agcctaagaa gaagaggaag gtttaaaagg
      241 gcgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agataactga
      301 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      361 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      421 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      481 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
      541 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
      601 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      661 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      721 gggcgaaaaa ccgtctatca
//
