LOCUS       sequence-99624-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha R C-D
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(189..194,195..212,213..219)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(339..358)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgtactgggg tggattacgg gtctcacacc cagattgtct tcaaagctga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc gcattaggca
      181 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      241 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      301 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      361 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
      421 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
      481 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
      541 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
      601 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
      661 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtgtca g
//
