LOCUS       sequence-99713-e         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, 
            Dietmann S, Hanna JH, Surani MA
  TITLE     SOX17 is a critical specifier of human primordial germ cell fate.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. 
            Epub 2014 Dec 24.
  PUBMED    25543152
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):253-68. doi"; pages: " 
            10.1016/j.cell.2014.12.013. Epub 2014 Dec 24"
COMMENT     Sequence Label: Human BLIMP1 sgRNA
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
       61 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
      121 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
      181 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
      241 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
      301 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
      361 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
      421 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
      481 tgttcatcca gctggtgc
//
