LOCUS       sequence-99718-e         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant 
            human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, 
            Dietmann S, Hanna JH, Surani MA
  TITLE     SOX17 is a critical specifier of human primordial germ cell fate.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. 
            Epub 2014 Dec 24.
  PUBMED    25543152
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):253-68. doi"; pages: " 
            10.1016/j.cell.2014.12.013. Epub 2014 Dec 24"
COMMENT     Sequence Label: Human T (BRACHYURY) sgRNA
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagccgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg
       61 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      121 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      181 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      241 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
      301 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
      361 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
      421 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
      481 acatgatcaa gttccgggg
//
