LOCUS       sequence-99893-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A direct fusion of sfGFP to the 24xGCN4_v1 peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-sfGFP-24xGCN4_v1
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..94
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             110..175
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             191..256
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             272..337
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             353..418
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             434..499
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             515..580
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             596..661
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             677..742
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             758..823
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 aggtcccggt ccgggaggtt ctggaggatt gttgcccaaa aactaccact tggagaacga
       61 ggtcgcaagg ttgaaaaaat tggtcggtga gcgtggcggc tcaggcggcc ttcttccaaa
      121 aaattatcat cttgaaaatg aagttgctag acttaaaaaa cttgttggag aaagaggcgg
      181 gagcggcggc ctgctgccca agaactacca cctggagaac gaggtcgccc gcctgaagaa
      241 gctggtcggc gagcgcggtg gttctggagg tttgctccca aagaactacc acttggaaaa
      301 cgaagtcgca agattgaaga agttggtcgg tgaaagagga ggaagcggag gattattacc
      361 taaaaattat catttggaaa atgaagttgc tagattgaag aagttggttg gagagagagg
      421 ggggtctgga ggactactac cgaaaaacta ccacctagag aacgaggtgg cccgactaaa
      481 aaagctagtg ggcgagcgcg gcggcagtgg cggcctcctc cccaagaatt accacctcga
      541 aaatgaagta gcaaggctca agaagctcgt aggagaaaga ggtggttccg ggggactcct
      601 tccaaaaaac tatcatcttg aaaacgaagt tgcacgtctt aaaaaacttg ttggcgaacg
      661 tggcggctct ggcgggctgc tgcccaagaa ttaccacctg gaaaatgaag tggcccgcct
      721 gaagaagctg gtgggcgaac gcggaggcag tggaggccta ctaccgaaga actaccacct
      781 agagaacgag gtggcacgcc taaagaagct agtgggcgag cgcggcggct cgggtggttt
      841 attacctaag aattatcatt tagaaaatga agtagctcga ttaaagaagt tagta
//
