LOCUS       sequence-99894-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A direct fusion of sfGFP to the 24xGCN4_v1 peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-sfGFP-24xGCN4_v1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..133
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             149..214
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 ttatcatctt gagaacgaag ttgctaggct caaaaagctg gttggagagc gcggcggctc
       61 aggcggtctg ttacccaaaa actaccactt ggaaaatgag gttgccagat tgaagaaact
      121 ggtcggcgag cgcggtggaa gtggaggact acttcctaaa aattatcatt tggagaacga
      181 agtcgctaga ctgaaaaagt tggttgggga acgaggaggg tctggcggca agctagcgat
      241 cggatcgatc gccgcggaac agcggcgcca tcatacacgc ggccgctaaa taaaccggtt
      301 gtacaggtac cggatcctaa ggtacctaat tgcctagaaa ggagcagacg atatggcgtc
      361 gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg ctgcagtatt
      421 cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac gatatggcgt
      481 cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg gctgcagtat
      541 tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga cgatatggcg
      601 tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct ggctgcagta
      661 ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag acgatatggc
      721 gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc tggctgcagt
      781 attcccgggt tcattagatc tcgcgaaggg cgaattcacg aggcgcgccc ggatcgcgat
      841 cgcaatctta ataactcgag tcta
//
