LOCUS       sequence-99945-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta F 1-2 RNAi
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             128..433
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     312..331
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    775..799
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
       61 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
      121 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
      181 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
      241 tgcacgtctg ctgtcagata aagtcccccg tgaactttac ccggtggtgc atatcgggga
      301 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg ttatcgggga
      361 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
      421 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacggtctca
      481 ggtgaatccg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
      541 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
      601 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
      661 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg accttgagtg
      721 acctaagcta gcctagtgtc gggcttgtct tctctggttt aaactatcag tgtttgacag
      781 gatatattgg cgggtaaacc taagagaaaa gagc
//
