LOCUS       sequence-99992-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid
            of the attB site. used for RMCE. CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: p3xP3-EGFP.vas-int.NLS
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    122..243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(159..178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     213..232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    250..283
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
       61 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
      121 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      181 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      241 ttagctagca taacttcgta taatgtatgc tatacgaagt tatgctagcc tgcagctggt
      301 tgtaggtgca gttgcgcttc ttgcacttgg cgcacttgag gagatcggtc ttggtgccct
      361 gcacggtggc cagctgggca tcgttgatgg cctccttgac gaacttctcg cgcagcttct
      421 tcatctcgtc gctggccatc tcctccggcg tcattttggc cagctgcttg gcagtgacgg
      481 cgccgcacat aaagttgccg cgcaatccag gattcttggg gtccttcaga ttggccacgc
      541 gtgacctaat gcgattcttg tacttcatat ccgtgttatt gaactcggag taaatggcat
      601 cctccagttc ggcggccatt tcctccggct caccgcatcc ctcgggcact tcgccaatct
      661 tcagtgcggt ggccagcatt tcgcggcatt tgatgcggac cgcatctgtc atgccaccgg
      721 agggaaacga ggtctgtgac gaggatgtcg agcctttctt ctctttatcc ttggatgagc
      781 tggatgaact ggacttatcc ttgcc
//
